Anti-P2RY2 Rabbit Polyclonal Antibody for WB, ELISA - P41231

Core Product Specifications and Parameters

Biological Background: P2Y purinoceptor 2 Function and Localization

• P2RY2 encodes the P2Y purinoceptor 2, also designated P2U purinoceptor 1 (P2U1), an ATP and UTP receptor.
• It is a G protein-coupled receptor (GPCR) that activates a phosphatidylinositol-calcium second messenger system.
• The receptor exhibits a high affinity for UTP and ATP, with the order UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP.
• P2RY2 localizes at the cell membrane and is a multi-pass transmembrane protein, belonging to the G protein-coupled receptor family.
• It is expressed in diverse tissues, including spleen, testis, kidney, liver, lung, heart, and brain.
• The protein is glycosylated and contains disulfide bonds, typical features of GPCRs.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal intracellular region (amino acids 278–377); the polyclonal antibody may recognize multiple epitopes within this domain.
• For Western blotting (WB), the predicted molecular weight is approximately 42 kDa; consider using denaturing conditions and validate with human or mouse samples known to express P2RY2.
• For ELISA, test the antibody with recombinant P2RY2 protein or peptide-coated plates; include proper controls for specificity.
• Cross-reactivity is indicated for human and mouse; if using other species, validate expression and antibody binding experimentally.
• As a polyclonal antibody, it is well-suited for detecting both native and denatured protein, but individual lot performance should be confirmed in the intended assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw-material procurement, offering validated antibodies like this anti-P2RY2 polyclonal to support purinergic signaling and GPCR research. Our portfolio extends to bulk ancillary reagents, rare target sourcing, and technical guidance, bridging the gap from early-stage discovery to clinical assay development.