Anti-P2RY2 Polyclonal Antibody for WB, ELISA - P41231

Core Product Specifications and Parameters

Biological Background: P2RY2 Function and Localization

• P2RY2 encodes the P2Y purinoceptor 2, also known as P2U purinoceptor 1, ATP receptor, or P2U1.
• It functions as a receptor for extracellular nucleotides ATP and UTP, with high affinity for both, and is coupled to G proteins that activate a phosphatidylinositol-calcium second messenger system.
• The affinity order is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP.
• P2RY2 belongs to the G protein-coupled receptor family and contains seven transmembrane helices.
• The receptor is localized at the cell membrane.
• It is expressed in various tissues including spleen, testis, kidney, liver, lung, heart, and brain.
• Post-translational modifications include disulfide bonds and glycosylation.
• It is involved in purinergic signaling and is classified under keywords such as receptor, transducer, and transmembrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal cytoplasmic region (residues 278-377), which is likely to be exposed only after membrane permeabilization; consider this when selecting fixation and permeabilization protocols for applications like immunofluorescence.
• For Western blot, a reducing denaturing gel is recommended to detect the linear epitope, and the observed band may appear around 42 kDa; however, glycosylation can cause a shift to a higher molecular weight.
• Validate the antibody in your specific sample system (human, mouse, or rat), as cross-reactivity is expected but may vary in expression levels across tissues.
• As a polyclonal antibody, batch-to-batch variability can occur; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering P2RY2 antibodies and related reagents to support GPCR signaling research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can help source rare target raw materials—all from a single, reliable platform. Whether you are developing diagnostic assays or conducting basic research, CamelBio streamlines procurement so you can focus on advancing your project.