Anti-P2RY14 Rabbit Polyclonal Antibody for WB, IHC-P, and ELISA - Q15391

Core Product Specifications and Parameters

Biological Background: P2RY14 Function and Localization

• P2RY14 encodes the P2Y purinoceptor 14, also known as GPR105 or the UDP-glucose receptor, a G protein-coupled receptor (GPCR).
• Functions as a specific receptor for UDP-glucose and other UDP-sugars, coupling to G-proteins to transduce extracellular signals; it is not activated by ATP, ADP, UTP, or other nucleotides.
• Belongs to the P2Y receptor family of GPCRs, characterized by seven transmembrane domains and involvement in diverse physiological processes.
• Localized primarily at the cell membrane, consistent with its role as a plasma membrane receptor.
• Demonstrates distinct tissue expression: highest in placenta, adipose tissue, stomach, and intestine; moderate in brain, spleen, lung, and heart; and lowest in kidney.
• Possesses structural features typical of class A GPCRs, including disulfide bonds, glycosylation sites, and a 3D structure (as indicated by UniProt keywords), with a calculated molecular weight of ~39 kDa.
• Plays potential roles in immune and inflammatory responses via UDP-glucose signaling, though precise physiological functions are still under investigation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 209–338 of human P2RY14, an intracellular region; this design may favor detection of denatured protein in western blotting.
• For IHC-P, consider optimizing fixation and antigen retrieval conditions to expose the intracellular epitope, especially in tissues like placenta or stomach where expression is high.
• Cross-reactivity with mouse and rat suggests sequence conservation in the immunogen region; validate the antibody in your model systems by comparing with appropriate controls.
• In WB, expect a band near 39 kDa; note that glycosylation or other modifications could shift the apparent molecular weight.
• ELISA can be used for quantification; include relevant standards and blank controls to ensure assay reliability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research labs, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like P2RY14 involved in GPCR signaling and nucleotide sensing, we help streamline procurement of rare-target raw materials and provide technical consultation from concept to clinic.