Anti-P2RY12 Polyclonal Antibody for WB, IF-P, ELISA - Q9H244

Core Product Specifications and Parameters

Biological Background: P2RY12 Function and Localization

• P2Y purinoceptor 12 (P2RY12), also known as P2Y12, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y(ADP), SP1999, and HORK3, is a G protein-coupled receptor belonging to the P2Y receptor family.
• It functions as a receptor for ADP and ATP, coupling to G proteins to inhibit the adenylyl cyclase second messenger system; it is not activated by UDP or UTP.
• P2RY12 is essential for normal platelet aggregation and blood coagulation, playing a critical role in hemostasis.
• The receptor is localized to the cell membrane and contains seven transmembrane domains, typical of GPCRs.
• Post-translational modifications include glycosylation and phosphorylation, as annotated in UniProt.
• Tissue expression is highest in platelets, with lower levels in the brain, spinal cord, and fetal brain; minimal expression is observed in lung, appendix, pituitary, and adrenal gland.
• The protein contains disulfide bonds and has been identified in multiple proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal intracellular region (amino acids 243–342), enabling detection of total P2RY12 protein in both denatured and native conditions.
• For Western blot, a band near 39 kDa is expected; consider using protease inhibitors and mild lysis conditions to preserve membrane protein integrity.
• In immunofluorescence (IF-P), permeabilization is required to access the intracellular epitope; optimize fixation and blocking steps for your cell type.
• Cross-reactivity with mouse and rat supports use in rodent models; validate expression in relevant tissues such as platelets or brain.
• ELISA can be employed for quantitative detection; pairing with a capture antibody may be necessary for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-P2RY12 polyclonal antibody supports research into platelet function, thrombosis, and hemostasis-related disorders. Through our extensive supply network, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and production.