Anti-P2RY1 Rabbit Polyclonal Antibody for WB and ELISA - P47900

Core Product Specifications and Parameters

Biological Background: P2RY1 Function and Localization

• P2RY1 (P2Y purinoceptor 1) is also known as the ADP receptor and purinergic receptor; it mediates cellular responses to extracellular adenine nucleotides.
• As a cell surface receptor, P2RY1 binds specifically to ADP, triggering activation of phospholipase C and mobilization of intracellular calcium ions (PubMed:25822790, PubMed:9038354, PubMed:9442040). Related references: PMID:25822790, PMID:9038354, PMID:9442040
• In platelets, ADP-mediated P2RY1 signaling induces platelet shape change and is critical for platelet aggregation and hemostasis (PubMed:9442040). Related references: PMID:9442040
• Localized at the cell membrane, P2RY1 is a G protein-coupled receptor with seven transmembrane domains and belongs to the purinergic receptor family.
• The protein is involved in blood coagulation and hemostasis, as indicated by its annotation keywords.
• Post-translational modifications include disulfide bonds and glycosylation, which are typical for GPCRs.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein corresponding to the C-terminal region (amino acids 274-373), suggesting potential reactivity with both native and denatured protein conformations.
• For Western blot, expect a band around 42 kDa; optimize sample preparation and antibody dilution to minimize non-specific binding.
• In ELISA applications, consider both direct antigen-coating and indirect capture formats; confirm reactivity in the relevant sample system.
• Reported cross-reactivity with mouse and rat indicates sequence conservation in the immunogen region; still verify reactivity in your species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our sourcing expertise covers rare targets such as GPCRs like P2RY1, supporting hemostasis and platelet function research. From concept to clinic, CamelBio streamlines your supply chain with one-stop access to high-quality reagents and technical consultation.