Anti-P2RX7 Polyclonal Antibody for WB, ELISA - Q99572

Core Product Specifications and Parameters

Biological Background: P2RX7 Function and Localization

• P2RX7 (P2X purinoceptor 7, also known as P2Z receptor or ATP receptor) is a ligand-gated nonselective cation channel belonging to the P2X receptor family.
• Channel activation by high concentrations of extracellular ATP triggers the influx of Na⁺ and Ca²⁺ and efflux of K⁺, and upon prolonged stimulation forms a large pore permeable to molecules up to ~900 Da.
  Related references: PMID:17483156 PMID:25281740 PMID:9038151 PMID:20453110 PMID:28235784
• In immune cells, P2RX7 functions as a danger sensor for extracellular ATP, promoting NLRP3 inflammasome assembly and the release of pro-inflammatory cytokines IL-1β and IL-18.
  Related references: PMID:26877061
• P2RX7 engagement can trigger programmed cell death pathways, including apoptosis and autophagy.
  Related references: PMID:21821797 PMID:23303206 PMID:28326637
• Alternative splicing generates isoforms with distinct functional properties, including an ion channel-only variant that lacks macropore function and non-functional variants.
• The receptor is localized to the plasma membrane and is widely expressed, with the highest levels found in brain and immune tissues.
• Post-translational modifications include N-linked glycosylation, phosphorylation, palmitoylation, and disulfide bond formation, which regulate receptor trafficking and function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 47–334 of human P2RX7, a region that may include extracellular domains common to multiple isoforms; anticipate detection of several isoform bands in Western blot.
• Due to glycosylation and potential receptor dimerization, the observed molecular weight in WB may exceed the predicted 69 kDa; validate in the appropriate sample system.
• For ELISA, consider optimizing coating buffer pH and blocking conditions to enhance specific signal.
• Given confirmed cross-reactivity with mouse and rat, the antibody can be used for these species, but species-specific validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to critical IVD raw materials—including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For P2RX7-related research in immunology and ATP signaling, we facilitate sourcing of high-quality antibodies, recombinant proteins, and rare-target raw materials. Partner with CamelBio to bridge your sourcing needs from concept to clinic.