Anti-P2RX5 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q93086

Core Product Specifications and Parameters

Biological Background: P2RX5 Function and Localization

• P2X purinoceptor 5 (P2RX5), also known as ATP receptor or purinergic receptor, is encoded by the P2RX5 gene. It belongs to the P2X family of ligand-gated ion channels.
• Functions as an ATP-gated nonselective cation channel, permeable to Na⁺, K⁺, Ca²⁺, and uniquely among P2X receptors, Cl⁻ ions (PubMed:12761352). May play a supporting role in the inflammatory response (By similarity). Related references: PMID:12761352
• Alternative splicing generates multiple isoforms, including a non-functional variant.
• Localizes primarily to the cell membrane as a multi-pass transmembrane protein.
• Highly expressed in the brain and immune system, suggesting roles in neuronal signaling and immune responses.
• Subject to post-translational modifications, including glycosylation and disulfide bond formation, which may influence channel function and stability.
• The UniProt canonical sequence predicts a molecular weight of ~49 kDa; however, the observed band in Western blotting is around 47 kDa, likely reflecting mature protein processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 341–422), covering a cytoplasmic domain. The antibody likely recognizes all isoforms containing this epitope; verify isoform specificity if needed.
• For Western blotting, expect a band near 47–55 kDa due to glycosylation. Validate detection in lysates from tissues or cells expressing P2RX5 (e.g., brain, immune cells).
• In immunofluorescence (IF/ICC or IF-P), permeabilize samples for intracellular epitope access, and anticipate plasma membrane staining.
• As a polyclonal antibody, cross-reactivity with other P2X family members is possible; consider using knockout or knockdown controls to confirm target specificity.
• Optimal working dilutions must be determined empirically for each application and experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement, offering validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents all in one place. This anti‑P2RX5 antibody equips researchers studying purinergic signaling, neuroinflammation, and ion channel pharmacology. Reach out to explore bulk supply options, custom sourcing for rare targets, and integrated technical support from concept to clinic.