Anti-P2RX4 Rabbit PolymAb® for WB, IF/ICC, IF-P, IHC-P, ELISA - Q99571

Core Product Specifications and Parameters

Biological Background: P2RX4 Function and Localization

• P2RX4 is a ligand-gated ion channel that is activated by extracellular ATP, forming a nonselective transmembrane pore permeable to sodium, potassium, and calcium (PubMed:9016352). Related references: PMID:9016352
• It plays essential roles in the immune system, including the initial steps of T-cell activation, Ca2+ microdomain formation, and promotion of Th17 cell differentiation via RORC expression (PubMed:35165166). Related references: PMID:35165166
• P2RX4 also participates in basal T-cell activity independently of TCR/CD3 stimulation, contributing to immune homeostasis.
• In the central nervous system, P2RX4 activation drives microglial motility through the PI3K/Akt pathway, implicating it in neuroinflammatory processes.
• The receptor localizes to the plasma membrane and has been detected in lysosomal membranes, where it may function as an ATP-gated cation channel.
• P2RX4 is a glycoprotein with multiple transmembrane helices, disulfide bonds, and undergoes alternative splicing, reflecting its complex regulation.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant protein corresponding to amino acids 55-338 of human P2RX4 (NP_110378.3), which spans the extracellular region. This design is suitable for targeting the extracellular domain in applications like WB, IHC-P, and IF/ICC.
• For Western blotting, P2RX4 migrates at approximately 43 kDa under reducing conditions. Optimize blocking and antibody dilution to minimize background.
• When performing immunofluorescence, consider using permeabilization methods appropriate for membrane proteins, as P2RX4 is a multi-pass transmembrane protein.
• Cross-reactivity is confirmed with human, mouse, and rat samples. Validate species specificity if used in other organisms.
• The antibody is recommended for ELISA, but perform titration to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a comprehensive range of IVD raw materials, including validated antibody pairs and monoclonal antibodies like this P2RX4 PolymAb®. With expertise in ion channel targets and immunological reagents, CamelBio supports your assay development from concept to clinical application. Rely on CamelBio for consistent supply of high-quality antibodies, ancillary reagents, and technical consulting services.