Anti-P2RX4 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q99571

Core Product Specifications and Parameters

Biological Background: P2RX4 Function and Localization

• P2RX4 is a member of the P2X purinergic receptor family and functions as an ATP-gated nonselective cation channel permeable to Na⁺, K⁺, and Ca²⁺ (PubMed:9016352). Related references: PMID:9016352
• It plays key roles in immunity and central nervous system physiology, including T-cell activation, Ca²⁺ microdomain formation, and basal T-cell activity (By similarity).
• P2RX4 promotes Th17 cell differentiation and activation via regulation of retinoic acid-related orphan receptor C (RORC) expression (PubMed:35165166). Related references: PMID:35165166
• Upon activation, it drives microglia motility through the PI3K/Akt pathway (By similarity).
• Could also function as an ATP-gated cation channel on lysosomal membranes (By similarity).
• The protein localizes to the cell membrane and lysosome membrane, consistent with its dual roles in signaling and intracellular ion balance.
• Post-translational modifications include glycosylation and disulfide bond formation; alternative splicing produces multiple isoforms.
• Keywords: ATP-binding, glycoprotein, ion channel, ligand-gated ion channel, receptor, transmembrane.

Experimental Guidance and Technical Tips

• The immunogen covers the large extracellular loop of P2RX4, suggesting suitability for detecting both native and denatured forms; consider optimizing conditions for each application.
• For western blot, a starting dilution range and appropriate positive/negative controls are recommended; the predicted molecular weight is ~43 kDa, though glycosylation may shift migration.
• For immunofluorescence on paraffin-embedded sections (IF-P), antigen retrieval and methanol fixation may improve epitope exposure.
• Cross-reactivity with mouse and rat has been reported; validate in the relevant sample system to confirm specificity.
• ELISA performance may be enhanced by using the antibody as a capture or detection reagent; consider checkerboard titrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials, including this anti-P2RX4 antibody for purinergic signaling, immunology, and neuroscience studies. In addition to validated antibody pairs and optimized monoclonals, we offer bulk ancillary reagents and specialized sourcing for rare-target raw materials. Contact us to discuss how our one-stop supply capabilities can support your project from concept to clinic.