Anti-P2RX4 Polyclonal Antibody for IF/ICC, IF-P, ELISA - Q99571

Core Product Specifications and Parameters

Biological Background: P2RX4 Function and Localization

• P2RX4 (P2X4) is a member of the P2X family of purinergic receptors that function as ligand-gated ion channels.
• It forms an ATP-gated nonselective cation channel permeable to Na+, K+, and Ca2+ (PubMed:9016352). Related references: PMID:9016352
• CTP acts as a weak partial agonist, while GTP and UTP are ineffective (By similarity).
• In the immune system, P2RX4 promotes the differentiation and activation of Th17 cells via regulation of RORC (PubMed:35165166). Related references: PMID:35165166
• It also participates in T-cell receptor-mediated and basal calcium signaling and microdomain formation (By similarity).
• In the central nervous system, P2RX4 activation drives microglial motility through the PI3K/Akt pathway (By similarity).
• Subcellularly, it localizes to the plasma membrane and may function as an ATP-gated channel on lysosomal membranes.
• Structurally, it features three transmembrane helices, extracellular disulfide bonds, and N-linked glycosylation; it binds ATP and is classified as a receptor and ion channel.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 111–211) corresponds to an extracellular loop region; thus, the antibody is well-suited for detection of surface-expressed P2RX4 in IF/ICC on non-permeabilized cells.
• For IF/ICC, use appropriate blocking (e.g., 5% normal serum from the secondary antibody host species) and validate staining by peptide competition or knockout controls.
• In ELISA, the synthetic peptide immunogen can serve as a positive control; plate coating with cell lysates from P2RX4-expressing tissues is recommended.
• For IF-P on paraffin sections, optimize antigen retrieval conditions and confirm cross-reactivity with rat tissue as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-P2RX4 antibody to support research into purinergic signaling, ion channel biology, and immune cell function. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your path from concept to clinical application.