Anti-P2RX3 Polyclonal Antibody for WB, ELISA - P56373

Core Product Specifications and Parameters

Biological Background: P2RX3 Function and Localization

• P2RX3, also known as P2X purinoceptor 3 or ATP receptor, is encoded by the P2RX3 gene in humans.
• It functions as an extracellular ATP-activated non-selective cation channel, as demonstrated by functional studies. Related references: PMID:10440098, PMID:27626375, PMID:29674445
• P2RX3 plays a critical role in sensory neurons, where its activation is essential for gustatory, nociceptive responses, visceral reflexes, and sensory hypersensitization.
• The channel is localized to the cell membrane, functioning as a transmembrane ligand-gated ion channel.
• It is permeable to calcium and magnesium ions and contains conserved disulfide bonds and glycosylation sites, consistent with its classification as a transmembrane glycoprotein receptor.
• The predicted molecular weight of the human P2RX3 protein is approximately 44 kDa.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 328-397) corresponds to the cytoplasmic C-terminal domain; thus, the antibody is expected to detect total P2RX3 in denaturing Western blots under reducing conditions.
• A band at approximately 44 kDa is predicted; include appropriate molecular weight markers for accurate size confirmation.
• Cross-reactivity with mouse and rat indicates potential utility in rodent models; however, validation in the specific tissue or cell system of interest is recommended.
• For ELISA applications, consider testing antibody binding to immobilized immunogen or native P2RX3; optimize coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop platform for IVD raw materials, technical support, and consulting, assisting you from early development to clinical application. Our P2RX3 polyclonal antibody is part of a broad portfolio supporting neuroscience and pain research. For bulk quantities, validated antibody pairs, or customized sourcing of ancillary reagents and rare targets, please reach out to our team.