Anti-P2RX2 Polyclonal Antibody for WB, ELISA - Q9UBL9

Core Product Specifications and Parameters

Biological Background: P2RX2 Function and Localization

• Protein identity and gene: P2RX2 encodes P2X purinoceptor 2, also known as the ATP receptor or purinergic receptor, a member of the P2X ligand-gated ion channel family.
• Core ion channel function: Functions as an ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium, and calcium, mediating cellular responses to extracellular ATP. Related references: PMID:10570044 PMID:31636190
• Role in hearing: In the inner ear, regulates sound transduction, auditory neurotransmission, outer hair cell electromotility, and K+ recycling, essential for normal hearing; mutations are linked to non-syndromic deafness DFNA41. Related references: PMID:23345450
• Synaptic signaling: Mediates excitatory postsynaptic responses in sensory neurons and synaptic transmission from neurons to smooth muscle, contributing to taste perception and peristalsis (by similarity).
• Subcellular localization: Localized primarily to the cell membrane, where it forms functional ion channels.
• Tissue distribution: Expressed in both the central and peripheral nervous systems, as well as in the pituitary gland.
• Post-translational modifications and structural features: Contains disulfide bonds, undergoes N-linked glycosylation, and is subject to alternative splicing; its three-dimensional structure has been experimentally determined.
• Disease association: Variants in P2RX2 cause autosomal dominant non-syndromic sensorineural deafness type DFNA41, highlighting its critical role in auditory function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 60–320 of human P2RX2, which covers a large extracellular domain, suggesting potential for detecting both native and denatured forms of the protein.
• For western blotting, expect a band near the predicted molecular weight of 52 kDa. Multiple bands or slight shifts may occur due to glycosylation or splice variants; validation in the relevant sample system is recommended.
• The antibody is expected to cross-react with mouse and rat P2RX2 based on sequence conservation, but reactivity in these species should be confirmed experimentally.
• For ELISA, consider pairing this polyclonal antibody with a matched monoclonal for detection; assay conditions (coating, blocking, detection) should be optimized in the specific matrix.

CamelBio: Your One-Stop Sourcing Bridge

As a leading one-stop IVD raw-material partner, CamelBio helps diagnostic manufacturers, labs, and research institutes move from concept to clinic with ease. This anti-P2RX2 polyclonal antibody supports research in hearing disorders, neurobiology, and ion channel signaling, and we complement it with validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and custom sourcing of rare-target raw materials. Reach out to discuss your specific procurement needs.