Anti-p19ARF Rabbit Polyclonal Antibody for WB, ELISA - Q64364

Core Product Specifications and Parameters

Biological Background: Tumor suppressor ARF Function and Localization

• Encoded by the Cdkn2a gene locus, p19ARF (Tumor suppressor ARF) is an alternative reading frame product and a key tumor suppressor, also known as cyclin-dependent kinase inhibitor 2A.
• Induces cell cycle arrest in G1 and G2 phases and acts as a potent tumor suppressor, as demonstrated in mouse models.
  Related references: PMID:8521522, PMID:9393858, PMID:15601844, PMID:17936562
• Binds to MDM2 and sequesters it in the nucleolus, blocking MDM2-mediated p53 degradation and thereby enhancing p53-dependent transactivation and apoptosis.
  Related references: PMID:10359817, PMID:9529248
• Also triggers G2 arrest and apoptosis independently of p53 by preventing the activation of cyclin B1/CDC2 complexes.
  Related references: PMID:15361884
• Localizes mainly to the nucleolus and nucleoplasm, with a mitochondrial isoform that may regulate autophagy and caspase-independent cell death.
• Binds transcription termination factor TTF1 and prevents its nucleolar localization, thereby inhibiting ribosome biogenesis.
  Related references: PMID:20513429
• Down-regulates BCL6-induced transcriptional repression and interacts with various proteins including E2F1, MYC, TOP1, and NPM1 to modulate transcription, rRNA processing, and ubiquitination pathways.
  Related references: PMID:15567177

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1-100) of mouse p19ARF. This region shares homology with human ARF, supporting observed cross-reactivity.
• For Western blot analysis, consider using nuclear or nucleolar-enriched lysates, as p19ARF predominantly localizes to these compartments. A band around 19 kDa is expected.
• ELISA performance may be assessed by peptide competition to confirm antibody specificity.
• When working with p19ARF, note that it can be expressed at low levels under normal conditions; induction of cellular stress or use of tumor cell lines may enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support cell cycle and tumor suppressor research. From concept to clinic, we streamline procurement so you can focus on assay development. For rare-target sourcing or customized workflows, contact us to explore tailored solutions.