Anti-p16 INK4A Polyclonal Antibody for WB - P51480

Core Product Specifications and Parameters

Biological Background: p16 INK4A Function and Localization

• p16 INK4A, also known as cyclin-dependent kinase inhibitor 2A (CDKN2A), is a tumor suppressor protein encoded by the Cdkn2a gene in mice. This gene undergoes alternative splicing to produce two structurally and functionally distinct proteins: p16 INK4A and p19 ARF, via an alternative reading frame.
• p16 INK4A functions as a negative regulator of cell proliferation by binding to cyclin-dependent kinases CDK4 and CDK6, preventing their association with cyclin D. This inhibition blocks the phosphorylation of retinoblastoma protein (Rb) and arrests the cell cycle at the G1/S checkpoint.
• The protein features four ankyrin repeat (ANK repeat) motifs that mediate high-affinity interactions with CDK4 and CDK6, forming the structural basis for its inhibitory activity.
• Subcellular localization studies indicate that p16 INK4A resides in both the cytoplasm and nucleus, enabling its regulatory roles in multiple compartments.
• Post-translational modifications include phosphorylation (noted as a Phosphoprotein), which may influence its stability and function.
• As a landmark tumor suppressor, loss or inactivation of p16 INK4A is frequently observed across a wide range of cancers, underscoring its critical role in maintaining normal cell cycle control.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against the N-terminal region (amino acids 1-100) of mouse p16 INK4A. It is expected to recognize the target in mouse, human, and rat samples based on reported cross-reactivity.
• For Western blotting, consider testing a range of antibody dilutions and selecting an appropriate detection method, as optimal conditions may vary by sample type and experimental system. The predicted molecular weight is approximately 17 kDa.
• In ELISA applications, evaluate the antibody’s performance with recombinant or native p16 INK4A under your established assay conditions to ensure satisfactory specificity and sensitivity.
• As a polyclonal antibody, acknowledge potential lot-to-lot variability; it is advisable to validate each new batch for consistency in your intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, technical services, and consulting, supporting the entire development journey from concept to clinic. Our p16 INK4A antibody exemplifies the high-quality reagents we provide for cell cycle and tumor suppressor research. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets — let us streamline your assay development with reliable, one-stop access.