Anti-p16-ARC/ARPC5 Polyclonal Antibody for WB, ELISA - O15511

Core Product Specifications and Parameters

Biological Background: p16-ARC/ARPC5 Function and Localization

• p16-ARC/ARPC5 is the official subunit 5 of the Actin-related protein 2/3 complex (Arp2/3 complex), a key regulator of actin polymerization.
• As a core component of the Arp2/3 complex, it mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). Related references: PMID:9230079
• Beyond its cytoplasmic role, the Arp2/3 complex also functions in the nucleus, promoting actin polymerization to regulate gene transcription and repair damaged DNA, including homologous recombination repair by facilitating the motility of double-strand breaks (DSBs) (PubMed:29925947). Related references: PMID:29925947
• The protein localizes to the cytoplasm, cytoskeleton, cell projections, and nucleus, reflecting its multi-faceted roles in cellular architecture and genome stability.
• Post-translational modifications include acetylation and ubiquitin-like conjugation, which may fine-tune its activity or interactions.
• Alternative splicing generates multiple isoforms, suggesting tissue-specific or condition-dependent regulation.
• The molecular weight is approximately 16 kDa, consistent with both its predicted and observed masses.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein encompassing amino acids 1–154 of human ARPC5, covering the full-length protein. This design supports detection of both native and denatured forms, making it suitable for WB and ELISA.
• For WB, expect a specific band near 16 kDa in appropriate cell or tissue lysates. We recommend using fresh lysates supplemented with protease inhibitors to preserve the low-molecular-weight protein.
• The antibody’s cross-reactivity with human, mouse, and rat indicates recognition of conserved epitopes. Validate the antibody in your species and application of interest by including appropriate positive and negative controls.
• In ELISA, titrate the antibody against the immunogen or native antigen to determine optimal working concentrations; typical rabbit polyclonal antibodies are used at 0.5–2 µg/mL for indirect ELISA.
• Given the Arp2/3 complex’s roles in actin dynamics and DNA repair, this antibody is a useful tool for studying cell migration, cytoskeletal reorganization, and genome maintenance. Consider probing nuclear fractions to explore its nuclear functions.
• If weak signal is observed, consider using an enhancer or extended exposure; the small protein size may result in lower transfer efficiency in WB, so optimize transfer conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by offering validated antibodies, matched pairs, and custom raw-material sourcing for the in-vitro diagnostics industry. This anti-p16-ARC/ARPC5 polyclonal antibody, targeting a conserved component of the Arp2/3 complex, supports research into actin-related cellular processes and DNA damage pathways—areas critical for oncology, immunology, and neurodegenerative disease panels. Beyond this product, we supply bulk ancillary reagents and tailor procurement solutions to accelerate your assay development from concept to clinic.