Anti-p150 CAF1 Rabbit Monoclonal Antibody for WB, ELISA - Q13111

Core Product Specifications and Parameters

Biological Background: p150 CAF1 (CHAF1A) Function and Localization

• p150 CAF1, also known as chromatin assembly factor 1 subunit A (CHAF1A), is a core component of the chromatin assembly factor 1 (CAF-1) complex.
• CAF-1 functions as a histone chaperone, facilitating the deposition of newly synthesized histones H3 and H4 onto replicating DNA during S-phase, a critical step in nucleosome assembly.
• CAF-1 also operates during DNA repair, ensuring proper chromatin structure restoration after DNA damage.
• The protein plays a role in heterochromatin maintenance in proliferating cells by targeting newly synthesized CBX proteins to heterochromatic replication foci.
• CHAF1A is localized exclusively to the nucleus, consistent with its role in DNA replication and chromatin dynamics.
• Post-translational modifications include phosphorylation, and the protein is subject to alternative splicing and ubiquitination, as highlighted by UniProt keywords.
• The predicted molecular weight is approximately 107 kDa (106,910 Da), consistent with the observed protein weight of ~107 kDa.

Experimental Guidance and Technical Tips

• For Western blot (WB), a reduced-denaturing condition is recommended given the predicted molecular weight of ~107 kDa; appropriate positive control lysates from human cell lines (e.g., HeLa, HEK293) should be included.
• The immunogen (a synthetic peptide within amino acids 100–200) may influence antibody performance; consider running a peptide competition assay to confirm specificity.
• For ELISA, optimize coating and blocking conditions using purified recombinant p150 CAF1 or cell lysates; human-specific reactivity should be validated.
• As CAF-1 expression is cell cycle-dependent, consider synchronizing cells to enrich for S-phase when performing assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material sourcing, connecting diagnostic manufacturers, labs, and researchers with high-quality reagents, including validated monoclonal antibodies like this anti-p150 CAF1 product. Specializing in chromatin biology and DNA replication/repair research tools, we offer a range of ancillary reagents, bulk supply options, and rare target sourcing to support your assay development and scale-up needs. Whether you're developing diagnostic kits for cell cycle or DNA damage markers, or need customized antibody pairs, our team facilitates reliable access to critical raw materials.