Anti-p150 CAF1 (CAF-1 p150 subunit) Rabbit Polyclonal Antibody for WB and ELISA - Q13111

Core Product Specifications and Parameters

Biological Background: p150 CAF1 Function and Localization

• Encoded by the CHAF1A gene, p150 CAF1 (also known as CAF-1 p150 subunit) is the largest subunit of the heterotrimeric chromatin assembly factor 1 (CAF-1) complex, which also includes p60 and p48 subunits.
• CAF-1 acts as a histone chaperone, specifically responsible for the deposition of newly synthesized histones H3 and H4 onto replicating DNA during the first step of nucleosome assembly; histones H2A/H2B are incorporated subsequently to complete the octamer.
• This assembly process is critical during DNA replication and repair, ensuring proper chromatin structure restoration; CAF-1 is also implicated in heterochromatin maintenance in proliferating cells by targeting newly synthesized CBX proteins to replication foci.
• p150 CAF1 localizes exclusively to the nucleus, consistent with its role in replication-coupled chromatin assembly.
• Post-translational modifications include phosphorylation (regulating its cell cycle-dependent activity) and ubiquitin-like conjugation, as highlighted by the keywords 'Phosphoprotein' and 'Ubl conjugation'.
• The protein undergoes alternative splicing, potentially producing isoforms with distinct functional properties.
• Structurally, p150 CAF1 has been studied via 3D-structure determination, revealing insights into its histone binding and complex assembly mechanisms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–200) of human p150 CAF1; antibody reactivity may be influenced by this domain’s accessibility. Consider validating detection in your specific sample system.
• For Western blot, the predicted molecular weight is approximately 107 kDa; ensure adequate protein extraction and transfer conditions for high-molecular-weight targets.
• For ELISA, standard optimization of coating antigen and antibody concentration is recommended.
• Given the antibody’s reported cross-reactivity with human p150 CAF1, confirm reactivity with related orthologs or isoforms if studying non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of IVD raw materials, offering validated polyclonal antibodies like this anti-p150 CAF1 antibody for chromatin biology and DNA repair research. We support diagnostic developers with one-stop access to monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing—bridging every stage from concept to clinic.