Anti-P Glycoprotein Rabbit pAb - P08183

Core Product Specifications and Parameters

Biological Background: P Glycoprotein Function and Localization

• ATP-dependent translocase ABCB1 (P-glycoprotein 1), also known as MDR1 or CD243, is encoded by the ABCB1 gene and belongs to the ATP-binding cassette (ABC) transporter family.
• Functions as an energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells, thereby mediating resistance to a broad range of chemotherapeutic agents. Related references: PMID:2897240 PMID:35970996 PMID:9038218
• Catalyzes the flop of phospholipids (phosphatidylcholine, phosphatidylethanolamine, glucosylceramides, sphingomyelins) from the cytoplasmic to the exoplasmic leaflet of the apical membrane, contributing to lipid homeostasis. Related references: PMID:2897240 PMID:35970996 PMID:8898203
• Primarily localizes to the apical cell membrane, with some cytoplasmic distribution, consistent with its role as a transmembrane transporter.
• Expressed in tissues with barrier functions: small intestine, liver, kidney, and brain. Related references: PMID:28408210
• Glycosylated and phosphorylated; keywords include ATP-binding, glycoprotein, phosphoprotein, transmembrane helix, and lipid transport.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 590-689, targeting an intracellular region; consider this when designing detection protocols.
• Suitable for Western blot (predicted band ~141 kDa), immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry, and ELISA. Validate in your specific sample type and optimize antibody dilution.
• Cross-reacts with human and mouse, enabling species-comparative studies.
• For phosphorylated or glycosylated forms, sample preparation may require appropriate phosphatase or deglycosylation controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like P Glycoprotein, we support diagnostic manufacturers and researchers in multidrug resistance and ABC transporter studies from early concept to clinical application. Our one-stop approach ensures access to rare-target raw materials and expert technical consulting.