Anti-P Glycoprotein Rabbit Monoclonal Antibody for WB, ELISA - P08183

Core Product Specifications and Parameters

Biological Background: P Glycoprotein Function and Localization

• P-glycoprotein (ABCB1) is an ATP-dependent translocase that effluxes a broad range of drug substrates and phospholipids across the membrane, playing a key role in multidrug resistance. Related references: PMID:2897240, PMID:35970996, PMID:9038218
• It catalyzes the outward translocation (flop) of various phospholipids, including phosphatidylcholine and sphingomyelin, from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Related references: PMID:8898203
• The protein is primarily localized at the apical cell membrane and also detected in the cytoplasm, consistent with a transporter that cycles between compartments.
• It is highly expressed in tissues with barrier functions such as small intestine, liver, kidney, and brain, influencing drug absorption and distribution.
• Structurally, it contains two nucleotide-binding domains (NBDs) and twelve transmembrane helices, with functional motifs for ATP-binding and phosphorylation, and is subject to N-linked glycosylation.
• Alternative splicing generates multiple isoforms, and the protein is clinically important as its dysfunction is linked to disease variants affecting drug pharmacokinetics.

Experimental Guidance and Technical Tips

• The immunogen was designed against a region spanning amino acids 393–710 of human P-glycoprotein, which corresponds to an intracellular nucleotide-binding domain (NBD1). This design may facilitate recognition of the native transporter across species.
• For WB, consider using reducing conditions and adequate protein loading, as P-glycoprotein is a high molecular weight (141 kDa) membrane protein.
• For ELISA, validate the antibody against recombinant or cell-expressed antigen. Given the reported cross-reactivity with mouse and rat, it may be suitable for preclinical studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and laboratories with one-stop access to raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For multidrug resistance and drug transport research, we support rare target raw-material sourcing and technical consulting to streamline your workflow from concept to clinic.