Anti-P Glycoprotein Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P08183

Core Product Specifications and Parameters

Biological Background: P Glycoprotein Function and Localization

• P glycoprotein 1 (ABCB1, also known as MDR1, PGY1, CD243) is encoded by the ABCB1 gene and belongs to the ATP-binding cassette (ABC) transporter superfamily.
• Its primary function is ATP-dependent drug efflux, actively expelling chemotherapeutic agents and hydrophobic xenobiotics from cells, a major mechanism of multidrug resistance in cancer. Related references: PMID:2897240 PMID:35970996 PMID:9038218
• The protein also acts as a phospholipid translocase, mediating the flop of phosphatidylcholine, phosphatidylethanolamine, β-D-glucosylceramides, and sphingomyelins from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Related references: PMID:8898203
• Subcellular localization is primarily at the cell membrane and apical cell membrane, with additional presence in the cytoplasm, consistent with its efflux function at polarized epithelial surfaces.
• In normal tissues, ABCB1 is expressed at high levels in the small intestine, liver, kidney, and brain, where it contributes to barrier function and xenobiotic excretion. Related references: PMID:28408210
• The protein is a glycoprotein and phosphoprotein with two nucleotide-binding domains and two transmembrane domains, featuring multiple transmembrane helices and ATP-binding sites.
• Genetic variations in ABCB1 are linked to disease variants, highlighting its clinical relevance in drug response and pathology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an intracellular segment (residues 603–692) of human P Glycoprotein; consider this when designing peptide blocking controls or evaluating epitope conservation across species.
• For Western blot analysis, expect a band at approximately 141 kDa; ensure adequate gel running time and transfer conditions for high‑molecular‑weight proteins.
• Reported cross‑reactivity includes human, mouse, and rat; validate performance in the intended sample system to confirm specificity.
• This antibody is recommended for WB, IF-P, IHC-P, and ELISA; optimal working concentrations should be empirically determined for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive supply chain for IVD raw materials, bridging diagnostic developers with rigorously validated antibody products like this anti-P Glycoprotein monoclonal antibody for multidrug resistance and cancer research. Our portfolio includes ready‑to‑use antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and customized sourcing of rare‑target raw materials. Let CamelBio accelerate your assay development with reliable, high‑quality immunological tools and expert technical consultation.