Anti-P-cadherin Rabbit Monoclonal Antibody for WB, IF, FC and ELISA - P22223

Core Product Specifications and Parameters

Biological Background: P-cadherin Function and Localization

• Cadherin-3, also known as P-cadherin, is a calcium-dependent cell adhesion protein belonging to the cadherin superfamily.
• Mediates homophilic cell-cell adhesion, playing a role in sorting heterogeneous cell types.
• Localizes to the cell membrane as a single-pass type I membrane protein.
• Expressed in select normal epithelial tissues and in some carcinoma cell lines.
• Associated with diseases such as ectodermal dysplasia and hypotrichosis; keywords also include vision-related phenotypes.
• Undergoes alternative splicing (as noted in UniProt), potentially generating multiple isoforms.
• Contains cadherin repeat domains and is subject to proteolytic cleavage on pairs of basic residues.
• Molecular weight is predicted at ~91.4 kDa; detected forms may vary due to modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 108-654, covering a large portion of the extracellular domain; this design likely yields antibodies targeting the N-terminal ectodomain.
• For Western blotting, observe bands around 87–91 kDa; use appropriate loading controls and validate in the relevant cell lines.
• In immunofluorescence applications (IF/ICC, IF-P), expect distinct membrane localization; co-stain with membrane markers to confirm specificity.
• For flow cytometry, the extracellular epitope may allow staining of live cells without permeabilization; optimize antibody concentration.
• The antibody is suitable for ELISA; consider its use as a capture or detection reagent in sandwich formats.
• Because cross-reactivity is limited to human, verify reactivity in your specific model system if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your centralized partner for IVD raw material sourcing, from discovery through clinical application. For P-cadherin and cell adhesion research, explore our portfolio of validated antibody pairs, stable monoclonal antibodies, and essential detection reagents. We also offer custom sourcing for rare targets to accelerate your diagnostic development.