Anti-OXSR1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95747

Core Product Specifications and Parameters

Biological Background: OXSR1 Function and Localization

• OXSR1 (oxidative stress-responsive 1), also known as OSR1, is a serine/threonine-protein kinase that acts as a key effector in the WNK-SPAK/OSR1 kinase cascade, regulating ion transport, cell volume, and blood pressure in response to hypertonic stress. Related references: PMID:16669787 PMID:18270262 PMID:21321328
• It specifically recognizes and binds RFXV/I motifs found in downstream substrates, including Na-K-Cl cotransporters (NKCC1, NKCC2), Na-Cl cotransporter (NCC), and inward rectifier potassium channels (Kir2.1, Kir2.3), modulating their activity and subcellular localization. Related references: PMID:16669787 PMID:17721439 PMID:29581290
• In hypertonic conditions, OXSR1 mediates regulatory volume increase by phosphorylation-dependent activation of NKCC1/NKCC2 and concurrent inhibition of K-Cl cotransporters KCC2/KCC3, leading to net ion influx. Related references: PMID:16669787 PMID:21321328 PMID:19665974
• In the kidney distal convoluted tubule, OXSR1 phosphorylates the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC, promoting sodium reabsorption and contributing to blood pressure homeostasis. Related references: PMID:18270262
• OXSR1 also participates in angiogenesis regulation in endothelial cells and phosphorylates additional substrates including PAK1, RELT, and PLSCR1, indicating broader signaling roles. Related references: PMID:23386621 PMID:25362046 PMID:14707132 PMID:16389068 PMID:28688764 PMID:22052202
• OXSR1 is a ubiquitously expressed cytoplasmic kinase, with its activity tightly regulated by upstream WNK kinases and post-translational modifications such as phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human OXSR1; this region is conserved across human, mouse, and rat, supporting the reported cross-reactivity. For species not listed, check sequence homology before use.
• For Western blotting, a band around 58 kDa is anticipated. Include appropriate positive and negative controls, and verify antibody specificity using lysates from OXSR1-expressing tissues or cell lines.
• For immunofluorescence/ICC, permeabilization is required for cytoplasmic detection. Fixation conditions (e.g., 4% PFA) should be optimized for your cell type. Co-stain with organelle markers to confirm localization.
• ELISA applications may require optimization of coating antigen and antibody titration; consider using the immunogen peptide as a positive control to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, research labs, and institutes with a comprehensive portfolio of IVD raw materials, covering enzymes, antibodies, and antigens. As a supplier of this OXSR1 polyclonal antibody, we facilitate access to validated reagents for the WNK-OSR1 signaling pathway, supporting research in ion transport, hypertension, and stress biology. Our one-stop model also offers bulk ancillary reagents, customized antibody production, and rare target sourcing to streamline your workflow from concept to clinic.