Anti-OXSM Rabbit Polyclonal Antibody for WB, ELISA - Q9NWU1

Core Product Specifications and Parameters

Biological Background: OXSM (3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial) Function and Localization

• OXSM encodes the mitochondrial 3-oxoacyl-[acyl-carrier-protein] synthase, also known as beta-ketoacyl-ACP synthase, which belongs to the beta-ketoacyl-ACP synthase family.
• It plays a role in the biosynthesis of lipoic acid and longer chain fatty acids required for optimal mitochondrial function.
• The protein localizes to the mitochondrion, consistent with its role in mitochondrial fatty acid metabolism.
• OXSM is widely expressed, with higher levels in tissues rich in active mitochondria, including heart, skeletal muscle, liver, and kidney.
• Post-translational modifications include acetylation, and the protein contains a predicted transit peptide for mitochondrial import.
• Alternative splicing generates multiple transcript variants.
• The protein's 3D structure has been determined, providing insights into its catalytic mechanism.
• It functions as an acyltransferase, mediating the transfer of acyl groups critical for lipid biosynthesis.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 49 kDa. Use appropriate loading controls and validate in the relevant sample system.
• The immunogen corresponds to amino acids 80–459 of human OXSM, covering a large portion of the protein; this may contribute to broad species cross-reactivity.
• As a polyclonal antibody, it recognizes multiple epitopes, which can enhance sensitivity but may necessitate careful validation for each new application or sample type.
• For ELISA, consider optimizing coating and detection conditions using the recombinant protein or native samples.
• Cross-reactivity with mouse and rat has been indicated; verify performance in these species using control lysates or recombinant proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD-grade antibodies and reagents. This OXSM polyclonal antibody supports studies on mitochondrial fatty acid biosynthesis and related metabolic pathways. In addition to this product, we offer validated antibody pairs, bulk ancillary reagents, and can assist with sourcing rare-target raw materials for your assay development needs.