Anti-OXR1 Rabbit Polyclonal Antibody for WB and ELISA - Q8N573

Core Product Specifications and Parameters

Biological Background: OXR1 Function and Localization

• OXR1, also designated TLDC3, CHEGDD, and Nbla00307, is a protein encoded by the OXR1 gene implicated in cellular defense against oxidative injury.
• It actively suppresses vacuolar-type ATPase (V-ATPase) function by triggering V-ATPase complex disassembly, a process essential for preventing excessive acidification of the Golgi lumen (PubMed:38593795).
  Related references: PMID:38593795
• Through interaction with the V-ATPase catalytic subunit ATP6V1A, OXR1 maintains optimal pH for Golgi-resident glycosylation enzymes (By similarity).
• OXR1 safeguards cells from oxidative damage (PubMed:11114193, PubMed:15060142) and exhibits neuroprotective effects under oxidative stress conditions (By similarity).
  Related references: PMID:11114193 PMID:15060142
• The protein distributes across multiple subcellular compartments including mitochondria, nucleus, nucleolus, and the Golgi apparatus where it associates with both cis- and trans-Golgi network membranes.
• UniProt annotates OXR1 with keywords such as Alternative splicing, Disease variant, Epilepsy, Intellectual disability, Membrane, Mitochondrion, Nucleus, Oxidation, Phosphoprotein, Stress response.
• The canonical isoform has a calculated molecular weight of approximately 98 kDa.

Experimental Guidance and Technical Tips

• For Western blot detection, use standard SDS-PAGE and transfer protocols; expect a band near 98–100 kDa. Validate specificity with appropriate positive and negative controls (e.g., OXR1-overexpressing lysates or knockout cell lines).
• In ELISA applications, titrate the antibody against recombinant OXR1 protein or the immunogen peptide to determine the optimal working concentration.
• The reported cross-reactivity with mouse and rat OXR1 makes this antibody suitable for rodent model studies; confirm reactivity in your species/tissue of interest before large-scale experiments.
• Due to the polyclonal nature, the antibody may recognize multiple OXR1 isoforms or degradation products; include protease inhibitors during sample preparation and consider using blocking peptides for confirmation of specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing for targets such as OXR1, providing optimized antibodies, validated pairs, and bulk ancillary reagents. With integrated technical services and consulting, we support diagnostic manufacturers, labs, and research institutes from feasibility to commercialization. Contact us to discuss your specific requirement and secure a stable supply of high-quality critical raw materials.