Anti-OXR1 Monoclonal Antibody for WB, IF-P, ELISA - Q8N573

Core Product Specifications and Parameters

Biological Background: OXR1 Function and Localization

• OXR1 functions as an oxidation resistance protein, providing protection from oxidative damage (PubMed:11114193, PubMed:15060142). Related references: PMID:11114193 PMID:15060142
• OXR1 inhibits the vacuolar-type ATPase (V-ATPase) by inducing disassembly of the V-ATPase complex (PubMed:38593795). Related references: PMID:38593795
• It protects the Golgi apparatus lumen from excess acidification by inhibiting V-ATPase ATP hydrolytic activity through interaction with the catalytic subunit ATP6V1A, maintaining optimal conditions for glycosylation enzymes (By similarity).
• The protein localizes to multiple subcellular compartments, including the mitochondrion, nucleus (nucleolus), and Golgi apparatus (cis-Golgi network membrane and trans-Golgi network membrane).
• OXR1 undergoes alternative splicing, producing multiple isoforms with varying molecular weights, consistent with the observed 24-98 kDa range.
• The protein is classified as a phosphoprotein and is associated with stress response, with disease variants linked to epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 705-874 of human OXR1, a region likely conserved across species, supporting cross-reactivity with human, mouse, and rat. Confirm sequence alignment for non-human targets.
• For Western blotting (WB), OXR1 isoforms migrate at 24-98 kDa; use appropriate molecular weight markers and validate band specificity in your sample system.
• In immunofluorescence (IF-P), consider possible localization to mitochondria, nucleus, nucleolus, and Golgi; optimize fixation/permeabilization to preserve organelle structure.
• For ELISA, validate assay conditions with recombinant OXR1 or relevant biological matrices to ensure sensitivity and specificity.
• Since the antibody is recommended for multiple applications, it is advisable to titrate and optimize for each experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, including this anti-OXR1 monoclonal antibody for oxidative stress and neuroprotection research. We supply validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare target raw materials, supporting diagnostic manufacturers from early concept to clinic.