Anti-OXGR1/GPR99 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q96P68

Core Product Specifications and Parameters

Biological Background: OXGR1/GPR99 Function and Localization

• OXGR1/GPR99 is a G protein-coupled receptor that primarily functions as a receptor for dicarboxylates and amino dicarboxylates, including itaconate, leukotriene E4 (LTE4), and alpha-ketoglutarate.
• In the respiratory epithelium, OXGR1 couples itaconate binding to GNA11 activation and intracellular calcium release, promoting mucociliary clearance of airborne pathogens, highlighting its role in innate immunity. Related references: PMID:36919698
• OXGR1 also serves as a high-affinity receptor for LTE4, a mast cell-derived mediator, and upon allergic inflammation, it triggers mucin release from pulmonary epithelium to protect against fungal allergens.
• In the kidney, OXGR1 mediates an intrarenal paracrine signaling pathway where it binds alpha-ketoglutarate to drive salt reabsorption and bicarbonate secretion in intercalated cells. Related references: PMID:15141213
• The receptor is localized to the cell membrane and contains key structural features such as transmembrane helices, disulfide bonds, and glycosylation sites, consistent with typical GPCR architecture.
• OXGR1 expression is detected in kidney and placenta, but absent from brain tissues, indicating tissue-specific regulatory roles in metabolism and immune response.

Experimental Guidance and Technical Tips

• Due to the synthetic peptide immunogen corresponding to an intracellular/extracellular loop region, the antibody may recognize both denatured (WB) and native (IF/ICC) conformations. Optimize fixation and permeabilization conditions for IF/ICC to preserve epitope accessibility.
• For Western blotting, use reducing conditions and validate in human, mouse, and rat lysates, given the reported cross-reactivity. The predicted band size is approximately 38 kDa; however, post-translational modifications such as glycosylation may result in a higher apparent molecular weight.
• For ELISA, consider coating with the immunogen peptide or recombinant protein to establish a standard curve. Include appropriate controls to confirm specificity.
• When studying tissue-specific expression, note that expression is higher in kidney and placenta but not detected in brain; use positive and negative tissue controls accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive catalog of IVD raw materials, including validated anti-OXGR1/GPR99 antibodies like this rabbit monoclonal antibody. Whether you need optimized antibody pairs, bulk ancillary reagents, or sourcing of rare targets such as GPCRs and innate immunity markers, CamelBio’s one-stop supply platform accelerates your development from concept to clinic.