Anti-OXCT2 Polyclonal Antibody for WB, IF-P, ELISA - Q9BYC2

Core Product Specifications and Parameters

Biological Background: OXCT2 Function and Localization

• OXCT2, also known as SCOT-t (testis-specific succinyl-CoA:3-oxoacid CoA-transferase), is a mitochondrial enzyme critical for ketone body catabolism.
• It catalyzes the transfer of CoA from succinate to acetoacetate, forming acetoacetyl-CoA, an essential step in utilizing ketone bodies for energy production.
• The enzyme operates via a covalent intermediate with CoA, proceeding through an unstable anhydride species formed between its carboxylate groups and the substrate (by similarity).
• OXCT2 is specifically expressed in the testis, suggesting a specialized role in ketone body metabolism within this tissue.
• It belongs to the 3-oxoacid CoA-transferase family and is localized to the mitochondria.
• The protein is annotated with keywords including Mitochondrion and Transferase, and has a predicted molecular weight of approximately 56 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 430-510), which may contribute to high specificity; consider validating with full-length recombinant protein if cross-reactivity is a concern.
• For Western blotting, a band around 56 kDa is expected; validate in the relevant sample system, keeping in mind possible mitochondrial targeting signal cleavage.
• In immunofluorescence (IF-P), mitochondrial localization can be confirmed by co-staining with established mitochondrial markers.
• For ELISA, the antibody may be suitable for detecting OXCT2 in testis tissue lysates; optimize coating and detection conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with a comprehensive, one-stop supply chain for IVD raw materials—from concept to clinic. For OXCT2-related ketone body metabolism studies, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Our expertise also encompasses rare target raw-material sourcing, ensuring you have the specialized tools needed for successful diagnostic innovation.