Anti-OXCT1 Rabbit Polyclonal Antibody for WB, ELISA - P55809

Core Product Specifications and Parameters

Biological Background: OXCT1 Function and Localization

• OXCT1 encodes the mitochondrial matrix enzyme succinyl-CoA:3-ketoacid coenzyme A transferase 1, also referred to as somatic-type SCOT (SCOT-s). It belongs to the 3-oxoacid CoA-transferase family.
• The enzyme catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues by transferring CoA from succinyl-CoA to acetoacetate, yielding acetoacetyl-CoA (PubMed:10964512). Related references: PMID:10964512
• Functional OXCT1 forms a homodimer; both subunits can form an enzyme-CoA thiolester intermediate, but only one subunit is competent to complete the transfer to the 3-oxo acid acceptor (By similarity).
• OXCT1 is localized in the mitochondrial matrix, consistent with its role in energy metabolism.
• The protein is highly expressed in heart, brain, kidney, skeletal muscle, and lung, but is absent from liver. Lower levels are detectable in leukocytes and fibroblasts.
• Post-translational modifications include phosphorylation; alternative splicing generates multiple transcript variants. Disease-associated variants have been reported (keywords: Phosphoprotein, Alternative splicing, Disease variant).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 261–520 of human OXCT1, a region conserved across human, mouse, and rat, supporting cross-species detection in these species.
• As a polyclonal antibody, it recognizes multiple epitopes, which can enhance WB signal but may also increase background; consider optimizing blocking and washing conditions.
• For ELISA, use the recombinant immunogen or purified native protein as coating antigen and perform appropriate antibody titrations.
• OXCT1 is a mitochondrial protein; enrichment of mitochondrial fractions from tissue or cell lysates may improve detection sensitivity in WB.
• Validate specificity using knockdown/knockout models or by comparing expression in heart (high OXCT1) versus liver (absent).

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CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies like this OXCT1 polyclonal antibody for metabolic and mitochondrial disorder research. Our supply capabilities span optimized monoclonal/polyclonal antibodies, antibody pairs, bulk ancillary reagents, and sourcing for rare targets, supporting your workflow from concept to commercialization.