Anti-OVOL2 Polyclonal Antibody for WB, ELISA - Q9BRP0

Core Product Specifications and Parameters

Biological Background: OVOL2 Function and Localization

• OVOL2 functions as a zinc-finger transcription repressor. Related references: PMID:19700410
• It suppresses keratinocyte cell cycling and terminal differentiation by directly repressing MYC and NOTCH1. Related references: PMID:19700410
• OVOL2 maintains epithelial identity by suppressing epithelial-to-mesenchymal transition (EMT) through repression of ZEB1.
• It positively regulates neuronal differentiation and is important for the correct development of primordial germ cells in embryos.
• OVOL2 plays dual roles in thermogenesis and adipogenesis to maintain energy balance, essential for brown/beige adipose tissue-mediated thermogenesis and limiting adipogenesis by blocking CEBPA activity.
• OVOL2 is expressed in testis, ovary, heart, and skeletal muscle. Related references: PMID:12213202
• It is expressed in the cornea but absent from the corneal endothelium, and mutations are linked to corneal dystrophy. Related references: PMID:26749309
• The protein localizes to the nucleus, contains zinc-finger domains, and undergoes alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 24–98) of human OVOL2; this sequence may be conserved in mouse and rat based on cross-reactivity data.
• For Western blotting, consider using nuclear extracts from testis, ovary, heart, or skeletal muscle where OVOL2 is expressed.
• For ELISA applications, validate coating conditions and detection range with the recombinant immunogen as a positive control.
• Cross-reactivity with mouse and rat has been noted, but independent validation in the relevant experimental system is recommended.
• As a polyclonal antibody, batch-to-batch consistency should be assessed for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, technical services, and consulting to support diagnostic manufacturers from concept to clinic. This anti-OVOL2 polyclonal antibody is part of our portfolio for epithelial biology and developmental research, complemented by validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing solutions. Partner with us to streamline your raw-material procurement and accelerate your assay development.