Anti-OVGP1 Rabbit Polyclonal Antibody for WB and ELISA - Q12889

Core Product Specifications and Parameters

Biological Background: OVGP1 Function and Localization

• OVGP1 (Oviduct-Specific Glycoprotein 1), also referred to as oviductin, mucin-9 (MUC9), estrogen-dependent oviduct protein, or oviductal glycoprotein, is a secreted glycoprotein primarily expressed in the oviduct.
• Encoded by the OVGP1 gene (GeneID: 5016), the protein contains a signal peptide and is classified as a mucin-like glycoprotein.
• OVGP1 localizes to cytoplasmic vesicles and secretory vesicles within oviductal epithelial cells, from where it is secreted into the oviductal fluid.
• The protein binds to the zona pellucida of oocytes in vivo, suggesting a role in regulating sperm-oocyte interaction and fertilization.
• OVGP1 may also be involved in early embryonic development by modulating the oviductal microenvironment and facilitating preimplantation embryo development.
• Expression of OVGP1 is estrogen-dependent, with levels fluctuating during the estrous cycle in response to hormonal changes.
• The protein structure includes disulfide bonds, contributing to its stability and function in the extracellular environment.
• Tissue specificity: predominantly expressed in the oviduct, with minimal or absent expression in other tissues.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 22–260 of human OVGP1, corresponding to the mature protein after signal peptide cleavage; this design favors recognition of the native secreted form.
• For Western blotting, consider that the theoretical molecular weight is ~75 kDa (UniProt: 75421 Da), but extensive glycosylation may result in a higher apparent molecular weight on SDS-PAGE.
• When performing ELISA, optimize coating conditions and antibody dilutions according to your specific assay format and sample type.
• Cross-reactivity with mouse and rat OVGP1 is supported by sequence homology in the immunogen region; validate reactivity in your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated antibodies for reproductive biology research. As a partner for your complete assay development, we offer optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like OVGP1 to support fertility and developmental biology applications.