Anti-OTX2 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - P32243

Core Product Specifications and Parameters

Biological Background: OTX2 Function and Localization

• OTX2 (Orthodenticle homolog 2) is a homeobox protein and transcription factor encoded by the OTX2 gene (GeneID: 5015). Alternative splicing generates multiple isoforms.
• As a developmental protein, OTX2 is crucially involved in brain and sense organ development, likely regulating key morphogenetic processes.
• OTX2 binds specifically to the bicoid/BCD target sequence (BTS: 5'-TCTAATCCC-3'), acting as a DNA-binding transcription factor.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Disease-associated variants of OTX2 are linked to microphthalmia and combined pituitary hormone deficiency (aliases CPHD6, MCOPS5).
• Identified in proteomics and reference proteome datasets, OTX2 is subject to post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 140–280 of human OTX2) may include conserved domains; verify sequence homology for expected cross-reactivity across species.
• For IHC-P, validate with appropriate positive and negative control tissues, as OTX2 expression is restricted to developing brain and eye.
• In IF/ICC, confirm the expected nuclear staining pattern consistent with OTX2’s subcellular localization.
• ELISA suitability suggests this polyclonal antibody can be used in quantitative assays; optimize coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For neurodevelopment and sensory organ research, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-OTX2, and bulk ancillary reagents. Our rare-target raw-material sourcing ensures you can secure critical components for your OTX2-related assays.