Anti-OTX2 Rabbit Monoclonal Antibody for WB, ELISA - P32243

Core Product Specifications and Parameters

Biological Background: OTX2 Function and Localization

• OTX2, also known as Orthodenticle homolog 2, is a homeobox protein that functions as a transcription factor.
• It is involved in the development of the brain and sense organs, and can bind to the bicoid target sequence (BTS: 5'-TCTAATCCC-3').
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• OTX2 undergoes alternative splicing, generating isoforms that may contribute to functional diversity.
• Mutations in OTX2 are linked to developmental disorders, including microphthalmia (as indicated by disease variant annotations in UniProt).
• It is a DNA-binding protein with a homeobox domain, classified under developmental proteins.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to residues 190-289 of human OTX2; this region may be conserved in mouse and rat, supporting observed cross-reactivity.
• For WB, consider the predicted molecular weight of ~32 kDa (based on UniProt) but note potential isoforms or post-translational modifications.
• Validate the antibody in the relevant species and sample type; cross-reactivity with mouse and rat is indicated but may require confirmation in your experimental system.
• For ELISA, use according to standard protocols and consider testing dilution ranges with your specific antigens.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers studying developmental transcription factors like OTX2, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic and research workflows.