Anti-OTX1 Monoclonal Antibody for WB and ELISA - P32242

Core Product Specifications and Parameters

Biological Background: OTX1 Function and Localization

• OTX1 (Homeobox protein OTX1, also known as Orthodenticle homolog 1) is a transcription factor belonging to the paired-like homeobox protein family.
• It likely plays a critical role in the development of the brain and sensory organs, such as the eyes.
• OTX1 can bind to the BCD target sequence (BTS) 5'-TCTAATCCC-3' to regulate gene expression.
• The protein localizes to the nucleus, consistent with its function as a DNA-binding transcription factor.
• It is predominantly expressed in the brain, and has been detected at the protein level in the anterior region of the neural fetal retina.
• Post-translational modifications include isopeptide bond formation and ubiquitination (Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide matching the N-terminal region (amino acids 1-100) of human OTX1, so the antibody likely recognizes epitopes in this domain. Consider this when designing experiments with truncated or mutant proteins.
• Given its cross-reactivity with mouse and rat, this antibody may be suitable for studies in common rodent models; validate in relevant tissue lysates.
• For Western blot, use appropriate nuclear extraction protocols to enrich OTX1, as it is a nuclear protein. Optimize blocking and antibody dilution conditions for your specific sample type.
• ELISA applications may benefit from using the immunogen peptide as a positive control to assess binding specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement experience for diagnostic developers and research laboratories, supplying high-quality IVD raw materials like this anti-OTX1 monoclonal antibody. From validated antibody pairs to bulk ancillary reagents and rare target sourcing, our one-stop platform supports your project from early-stage research to clinical application. Leverage our expertise in neuroscience and developmental biology markers to accelerate your assay development.