Anti-OTUD4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q01804

Core Product Specifications and Parameters

Biological Background: OTUD4 Function and Localization

• OTUD4, also known as HIV-1-induced protein HIN-1, is a deubiquitinase that hydrolyzes the isopeptide bond between ubiquitin and lysine residues on target proteins (PubMed:23827681, PubMed:25944111, PubMed:29395066).
  Related references: PMID:23827681 PMID:25944111 PMID:29395066
• Upon phosphorylation at Ser-202 and Ser-204, OTUD4 specifically deubiquitinates Lys-63-polyubiquitinated MYD88 adapter protein via IL-1 receptor and Toll-like receptor pathways, attenuating NF-kB-dependent inflammatory transcription (PubMed:29395066).
  Related references: PMID:29395066
• Independently of its catalytic activity, OTUD4 serves as a scaffold to assemble USP7 and USP9X deubiquitinases, stabilizing the alkylation repair enzyme ALKBH3 and promoting repair of alkylated DNA lesions (PubMed:25944111).
  Related references: PMID:25944111
• The protein localizes to both the cytoplasm and nucleus, consistent with its roles in immune signaling and DNA repair.
• OTUD4 undergoes post-translational modifications including phosphorylation (at least at Ser-202/Ser-204) and acetylation, and is subject to alternative splicing.
• The full-length protein has a calculated molecular weight of approximately 124 kDa (UniProt Q01804).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200-500 of human OTUD4, a central region that may detect endogenous OTUD4 in multiple application formats.
• For Western blotting, the full-length OTUD4 protein runs at approximately 124 kDa; a 6-8% SDS-PAGE gel and appropriate transfer conditions are recommended.
• For immunofluorescence/ICC, OTUD4 exhibits both cytoplasmic and nuclear localization; permeabilization conditions should be optimized to detect nuclear pools.
• ELISA experiments may be performed using the recombinant immunogen or cell lysates; consider validating with a known positive control.
• Cross-reactivity is expected with human, mouse, and rat OTUD4 based on sequence homology; however, performance in each species should be confirmed experimentally.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and it is advisable to include appropriate positive and negative controls in all assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive procurement partner for IVD raw materials, connecting diagnostic developers with high-quality antibodies and reagents. This anti-OTUD4 polyclonal antibody supports research into innate immune signaling, deubiquitination, and DNA damage repair. Explore our catalog for validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare target raw materials.