Anti-OTP Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q5XKR4

Core Product Specifications and Parameters

Biological Background: Homeobox protein orthopedia (OTP) Function and Localization

• OTP (Orthopedia) is a homeobox-containing transcription factor encoded by the OTP gene, playing a key role in early development and cell differentiation.
• It is probably involved in the specification and differentiation of hypothalamic neuroendocrine cells, as indicated by functional studies in model organisms.
• The protein localizes to the nucleus, where it binds DNA through its homeobox domain to regulate target gene expression.
• OTP is classified as a developmental protein associated with neurogenesis, highlighting its critical function in neuronal lineage commitment.
• Additional keywords such as "Differentiation", "DNA-binding", and "Transcription regulation" underscore its role as a transcriptional regulator in embryonic development.
• While post-translational modifications and interaction partners remain largely unexplored, OTP has been identified in high-throughput proteomics studies, suggesting it may be part of broader regulatory networks.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (aa 1–130) of human OTP; sequence alignment indicates high conservation in mouse and rat, consistent with the reported cross-reactivity.
• For Western blotting (WB), the predicted molecular weight is ~34 kDa; consider using nuclear extracts or tissue lysates from hypothalamus or pituitary for optimal detection.
• In immunohistochemistry (IHC-P), nuclear staining is expected; we recommend testing multiple antigen retrieval methods and titrating the antibody to minimize background.
• This polyclonal antibody can be used in ELISA for the detection of recombinant or endogenous OTP; however, batch-to-batch consistency should be verified when switching lots.
• As with all polyclonal antibodies, validation in your specific sample system and application is essential to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-OTP polyclonal antibody is ideal for research on hypothalamic neuroendocrine differentiation and related transcription factor pathways. Whether you need ready-to-use reagents or custom development support, CamelBio offers tailored supply solutions from concept to clinic, helping you accelerate assay development and product commercialization.