Anti-OTOF Polyclonal Antibody for WB, ELISA - Q9HC10

Core Product Specifications and Parameters

Biological Background: Otoferlin Function and Localization

• Otoferlin (Fer-1-like protein 2) is a multi-isoform protein; isoform 1 and 3 are expressed in adult brain, while isoform 2 is found in fetus and adult brain, heart, placenta, skeletal muscle, and kidney.
• Functions as a key calcium sensor at ribbon synapses of cochlear inner hair cells (IHCs), triggering synaptic vesicle exocytosis and neurotransmitter release by interacting with presynaptic SNARE proteins in a calcium-dependent manner.
• Also essential for synaptic exocytosis in immature outer hair cells (OHCs) and may play a role in endosome recycling.
• Belongs to the ferlin family of proteins, characterized by multiple C2 domains that mediate calcium-dependent membrane interactions.
• Subcellularly localizes to the cytoplasmic vesicle, synaptic vesicle membrane, basolateral cell membrane, endoplasmic reticulum membrane, Golgi apparatus membrane, presynaptic cell membrane, cell membrane, and trans-Golgi network.
• Contains transmembrane regions and a signal-anchor, anchoring it to membranes of synaptic vesicles and the presynaptic plasma membrane.
• Associated with genetic deafness: mutations cause autosomal recessive non-syndromic hearing loss (DFNB9) and auditory neuropathy spectrum disorder.
• Post-translational modifications and structural features include metal-binding, coiled-coil regions, and multiple C2 domains for calcium binding, crucial for synaptic function.

Experimental Guidance and Technical Tips

• For WB, due to the large protein size (~227 kDa), use low-percentage SDS-PAGE gels and extended transfer times; verify in appropriate human tissue lysates (e.g., brain, cochlear tissue).
• For ELISA, the immunogen region (N-terminal 1-300) may be used to capture or detect otoferlin; consider pairing with another antibody targeting a distinct epitope for sandwich ELISA.
• Validate antibody specificity in the relevant sample system, as otoferlin expression is tissue-specific and isoforms vary; human brain lysates or transfected cell lines are suitable positive controls.
• Since otoferlin is a synaptic vesicle protein, subcellular fractionation may enrich the signal in membrane fractions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop sourcing for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. For otoferlin-related hearing and deafness research, CamelBio offers validated antibodies, optimized monoclonals/polyclonals, bulk ancillary reagents, and rare target sourcing. Partner with us to streamline your auditory neuroscience assay development.