Anti-OsTT1 Rabbit Polyclonal Antibody for WB, ELISA - Q10KF0

Core Product Specifications and Parameters

Biological Background: OsTT1 (Proteasome subunit alpha type-2) Function and Localization

• OsTT1 (also known as PAB1, OsPAB1) encodes the 20S proteasome subunit alpha type-2 in Oryza sativa (rice), a core component of the 26S proteasome complex.
• The proteasome is a multicatalytic proteinase complex that cleaves peptides with broad specificity, recognizing Arg, Phe, Tyr, Leu, and Glu residues adjacent to the leaving group at neutral or slightly basic pH.
• It exhibits ATP-dependent proteolytic activity, playing a central role in intracellular protein degradation and turnover.
• OsTT1 belongs to the alpha-type subunit family of the 20S proteasome, which forms the outer rings of the barrel-shaped proteasome core particle.
• Subcellularly, the protein localizes to both the cytoplasm and the nucleus, consistent with the proteasome's function in degrading regulatory and misfolded proteins in these compartments.
• As a reference proteome component, OsTT1 is essential for maintaining protein homeostasis and regulating diverse cellular processes in rice.

Experimental Guidance and Technical Tips

• The immunogen was designed against a central region (aa 135–235) of OsTT1, which may provide specificity for the target protein.
• For Western blot, the predicted molecular weight is ~26kDa; consider using appropriate gel percentage and transfer conditions.
• ELISA can be used to detect the recombinant protein or endogenous OsTT1 in rice extracts; consider optimizing coating conditions and antibody dilutions.
• Cross-reactivity is reported for Oryza sativa; validate in the relevant sample system to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, supporting the development of assays and research tools with a comprehensive portfolio of antibodies, antigens, and ancillary reagents. This anti-OsTT1 antibody is designed for plant proteasome research and agricultural diagnostics. Our offerings include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk reagents, and rare target sourcing to accelerate your project from concept to clinic.