Anti-Osteopontin Monoclonal Antibody for WB, ELISA - P10451

Core Product Specifications and Parameters

Biological Background: Osteopontin Function and Localization

• Osteopontin (OPN), encoded by the SPP1 gene (also known as BNSP, Bone Sialoprotein 1, ETA-1), is a secreted glycoprotein belonging to the SIBLING family of proteins.
• It functions as a major non-collagenous bone matrix protein, binding tightly to hydroxyapatite and playing an integral role in biomineralization and cell-matrix adhesion.
• Acts as a cytokine, enhancing production of interferon-gamma and interleukin-12 while reducing interleukin-10, thereby promoting type I immunity.
• Primarily secreted, OPN is found in various body fluids and tissues, including bone, plasma, cerebrospinal fluid, and urine.
• Detected at the protein level in cerebrospinal fluid and urine, and highly expressed in bone. Related references: PMID:25326458 PMID:36213313 PMID:37453717
• Post-translational modifications include glycosylation, phosphorylation, and sialic acid attachment, contributing to its functional diversity.
• Alternative splicing generates multiple isoforms with distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 200-300 of human Osteopontin (P10451), targeting the C-terminal region. For Western blot, expect a band at approximately 35 kDa; consider optimizing sample preparation for secreted proteins, such as using serum-free conditioned media or concentrated biological fluids.
• For ELISA applications, pair this detection antibody with a suitable capture antibody validated for human Osteopontin; titrate both antibodies to determine optimal concentrations for your specific assay format.
• Although validated in human samples, cross-reactivity with other species is not reported; if extending to other species, sequence alignment is recommended and experimental validation should be performed.
• Use appropriate positive and negative controls, such as recombinant human Osteopontin protein and knockout cell lysates, to confirm specificity in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This Osteopontin monoclonal antibody supports research in bone biology, mineralization, and immune regulation, and our supply chain ensures consistent quality for your assay development and production needs.