Anti-Osteoglycin (OGN) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P20774

Core Product Specifications and Parameters

Biological Background: Osteoglycin (OGN) Function and Localization

• Osteoglycin (OGN), also known as mimecan or osteoinductive factor (OIF), is a secreted proteoglycan belonging to the small leucine-rich repeat proteoglycan (SLRP) family.
• Encoded by the OGN gene, this protein contains leucine-rich repeats and is characterized by a signal peptide for secretion.
• OGN plays a role in bone biology by inducing bone formation, particularly in synergy with transforming growth factors TGF-beta-1 or TGF-beta-2.
• It is predominantly expressed in bone tissue.
• Subcellularly, OGN is localized to the extracellular matrix and secreted into the extracellular space.
• Post-translational modifications include glycosylation and disulfide bond formation, consistent with its function as a proteoglycan.
• Keywords: direct protein sequencing, glycoprotein, growth factor, proteoglycan, secreted, signal, extracellular matrix, leucine-rich repeat, disulfide bond.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-298 of human OGN, covering the mature protein sequence; consider this when evaluating cross-reactivity with other species or isoforms.
• This antibody is suitable for Western blot (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA; optimize dilutions for each application in the relevant sample system.
• Due to its cross-reactivity with human, mouse, and rat, it can be employed in studies across these species; validate in the intended sample type.
• The predicted molecular weight of OGN is ~34 kDa; confirm the observed band in WB under reducing conditions.
• For bone-related studies, consider using appropriate positive control tissues such as bone or osteoblast-derived samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined source for IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies for targets such as bone-related proteoglycans. Our portfolio supports applications from biomarker discovery to assay development, with bulk ancillary reagents and rare target raw-material sourcing available to accelerate your projects.