Anti-Osteocalcin/OCN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P02818

Core Product Specifications and Parameters

Biological Background: Osteocalcin Function and Localization

• Osteocalcin, also known as Bone Gla protein (BGP) or gamma-carboxyglutamic acid-containing protein, is encoded by the BGLAP gene and represents 1-2% of total bone protein. Related references: PMID:3019668 PMID:6967872
• The carboxylated form acts as a negative regulator of bone formation and binds strongly to apatite and calcium in a gamma-carboxylation-dependent manner, which is essential for bone metabolism. Related references: PMID:6967872 PMID:39880952
• The uncarboxylated form functions as a hormone that regulates energy metabolism by promoting pancreatic beta-cell proliferation, insulin secretion, and sensitivity.
• This hormonal form also promotes testosterone production in Leydig cells via GPRC6A and supports brain development by acting on neurons through GPR158, where it prevents apoptosis and influences neurotransmitter synthesis.
• Osteocalcin is a secreted protein that undergoes gamma-carboxylation of specific glutamate residues, a modification critical for its structural and regulatory roles.
• Key biological processes associated with Osteocalcin include biomineralization, calcium binding, and hormonal signaling, highlighting its multifaceted role in bone and systemic physiology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human Osteocalcin; it may detect both carboxylated and uncarboxylated forms—validate in your assay system.
• For Western blot, the predicted molecular weight is approximately 11 kDa, though post-translational modifications like gamma-carboxylation can influence electrophoretic mobility; consider using appropriate controls.
• In immunofluorescence (IF/ICC), since Osteocalcin is secreted, optimize fixation and permeabilization conditions to detect matrix-associated or intracellular protein pools.
• For ELISA, confirm cross-reactivity with human, mouse, and rat samples using species-matched standards and include relevant blocking steps to ensure specificity.
• Due to the protein's dual role as a bone matrix component and circulating hormone, sample preparation (e.g., extraction from tissue vs. serum/plasma) should be tailored to the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined, one-stop source for IVD raw materials, from initial development to clinical application. This anti-Osteocalcin antibody supports bone metabolism and metabolic research, and CamelBio can supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw materials. For all your procurement needs, CamelBio serves as a reliable partner to accelerate your project.