Anti-Osteocalcin (BGLAP) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P02818

Core Product Specifications and Parameters

Biological Background: Osteocalcin Function and Localization

• Osteocalcin, encoded by the BGLAP gene, is also known as Bone Gla protein (BGP) or Gamma-carboxyglutamic acid-containing protein. It is a small secreted protein with a molecular weight of approximately 11 kDa.
• The carboxylated form of osteocalcin is a major organic component of the bone matrix, constituting 1-2% of total bone protein. It binds strongly to apatite and calcium, which is essential for bone metabolism and mineralization. Related references: PMID:3019668, PMID:6967872, PMID:39880952
• Acts as a negative regulator of bone formation, limiting bone formation without impairing bone resorption or mineralization.
• The uncarboxylated form functions as a hormone, regulating energy metabolism by promoting pancreatic beta-cell proliferation, insulin secretion, and sensitivity. It also supports male fertility by enhancing testosterone production via GPRC6A in Leydig cells.
• In brain development, osteocalcin crosses the blood-brain barrier and binds to GPR158 on neurons, preventing apoptosis, promoting monoamine neurotransmitter synthesis, and inhibiting GABA production. Maternal osteocalcin also crosses the placenta for fetal brain development.
• Post-translational gamma-carboxylation of glutamic acid residues is critical for its calcium-binding and bone-related functions. The protein contains a disulfide bond and is secreted after signal peptide cleavage.
• Keywords from UniProt include biomineralization, calcium, hormone, and secreted, reflecting its dual roles in bone and systemic physiology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human osteocalcin, which may allow detection of both carboxylated and uncarboxylated forms; verify specificity if required.
• Given the polyclonal nature, consider validating the antibody in your specific sample system and include appropriate positive and negative controls.
• Suitable for WB, IF/ICC, and ELISA; titration and optimization of working dilutions are recommended as reactivity may vary.
• Cross-reactivity with mouse and rat is indicated; this can be advantageous for translational studies but should be confirmed under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This osteocalcin antibody supports bone metabolism and hormone research, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Let CamelBio be your sourcing bridge for rare targets and specialized IVD raw materials.