Anti-OsTB1 Rabbit Polyclonal Antibody for WB - Q8LN68

Core Product Specifications and Parameters

Biological Background: OsTB1 Function and Localization

• OsTB1 (TCP8, FC1) is a transcription factor belonging to the TCP family, also known as TEOSINTE BRANCHED 1 homolog in rice (Oryza sativa subsp. japonica).
• It functions as a negative regulator of lateral branching, with expression in axillary buds inhibiting bud outgrowth. Related references: PMID:12581309 PMID:20547591
• OsTB1 acts as an integrator of multiple signaling pathways, working partially downstream of strigolactones to suppress tillering. Related references: PMID:20547591
• The protein binds to MADS57, suppressing its negative regulation of D14, thereby balancing D14 expression for tillering control. Related references: PMID:23463009
• Subcellularly, OsTB1 localizes to the nucleus, consistent with its role as a transcription factor.
• Tissue expression includes axillary buds, shoot apical meristem, young leaves, vascular tissues, and tips of crown roots. Related references: PMID:17655651 PMID:20547591
• Keywords: DNA-binding, growth regulation, transcription regulation, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 270-388) of OsTB1, which may include a conserved TCP domain; consider verifying reactivity in your specific rice cultivar.
• For WB, use nuclear extracts or total protein from axillary bud-enriched tissues; the predicted molecular weight is approximately 42 kDa.
• Validate antibody specificity with knockdown or knockout controls, and optimize sample preparation to maintain protein stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized supplier of IVD raw materials, providing validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. Our portfolio supports research in plant developmental biology, focusing on transcription factors and signaling pathways like those involved in tillering. Contact us for customized sourcing of antibodies, antigens, and materials tailored to your assay development needs.