Anti-OsRDR6/SHL2 Rabbit Polyclonal Antibody for WB & ELISA - Q8LHH9

Core Product Specifications and Parameters

Biological Background: OsRDR6 Function and Localization

• OsRDR6, encoded by the SHL2 gene, is also known as SHOOTLESS2. It is a probable RNA-dependent RNA polymerase in rice (Oryza sativa subsp. japonica).
• The protein plays a central role in the RNA silencing pathway, likely generating small interfering RNAs (siRNAs) that guide sequence-specific gene silencing.
• OsRDR6 is essential for shoot apical meristem (SAM) initiation and maintenance, as well as leaf polarization. It functions through the trans-acting siRNA (ta-siRNA) pathway.
• This ta-siRNA pathway modulates the expression of auxin response factor genes, including ARF2, ARF3, ARF4, ARF14, and ARF15, thereby influencing plant development.
• As a nucleotidyltransferase and RNA-directed RNA polymerase, OsRDR6 binds RNA and catalyzes the synthesis of complementary RNA strands, a key step in amplification of silencing signals.
• The protein is classified under RNA-mediated gene silencing and transferase activities, reflecting its enzymatic function in post-transcriptional gene regulation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 963–1218, a C-terminal region of OsRDR6. For Western blot, consider loading appropriate rice tissue or cell lysates expressing endogenous OsRDR6, and note the predicted molecular weight of approximately 137 kDa.
• For ELISA applications, the antibody can be used to detect recombinant or native OsRDR6; validate optimal coating and detection conditions in your assay system.
• Cross-reactivity is reported for Oryza sativa; cross-reactivity with other species has not been tested. Verify specificity in any non-rice experimental models.
• Include appropriate positive and negative controls (e.g., shl2 mutant tissues or RNAi knockdown lines) to confirm band identity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents for various target research areas. For studies focusing on plant developmental biology and RNA silencing mechanisms, CamelBio offers specialized sourcing of rare-target reagents like OsRDR6 antibodies, ensuring traceable quality and reliable supply chains. Our team provides technical consultation to help you integrate these materials seamlessly into your assay development workflows.