Anti-OsRACK1A Polyclonal Antibody for WB and ELISA - P49027

Core Product Specifications and Parameters

Biological Background: OsRACK1A Function and Localization

• OsRACK1A, also known as RACK1z, GPB-LR, RWD, or Receptor for activated C kinase 1A, is a small ribosomal subunit protein in rice (Oryza sativa) belonging to the WD-repeat protein family.
• It functions as a component of the RACK1 regulatory proteins, playing a key role in innate immunity by interacting with multiple proteins in the RAC1 immune complex.
• Acts as a positive regulator of reactive oxygen species (ROS) production and is required for resistance against rice blast (Magnaporthe grisea) infection.
• Localizes to the cytoplasm and cell membrane.
• Widely expressed across tissues.
• Contains WD repeats and is classified as a ribosomal protein, transducer, and plant defense-related protein.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against a recombinant protein covering amino acids 1-334, it may exhibit broad epitope recognition. Validate in the relevant sample system (Oryza sativa) using appropriate positive and negative controls.
• For western blot (WB), a band around 36 kDa is expected. Consider using whole-cell lysates from rice leaf or other tissues where OsRACK1A is expressed.
• ELISA applications can be optimized by coating with recombinant OsRACK1A or plant extract, depending on assay design.
• Due to potential sequence homology among plant RACK1 proteins, cross-reactivity should be verified if used in non-rice species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to provide a comprehensive range of IVD raw materials, including validated antibodies for plant innate immunity research. This anti-OsRACK1A polyclonal antibody supports studies on rice defense mechanisms and can be integrated into assay development. As a sourcing bridge, CamelBio also offers bulk ancillary reagents and rare target antibodies to streamline your R&D workflow.