Anti-OsNPR1 Polyclonal Antibody for WB and ELISA - Q9FDY4

Core Product Specifications and Parameters

Biological Background: OsNPR1 Function and Localization

• OsNPR1 (NPR1 homolog 1, OsNH1) is a rice protein containing a BTB/POZ domain, ankyrin repeats, and a zinc-finger, functioning in plant defense signaling.
• Acts as a salicylic acid receptor and substrate adapter for the CUL3-RBX1 E3 ubiquitin ligase complex, targeting proteins for ubiquitin-dependent degradation.
• Functions as a transcription cofactor: represses gene expression via WRKY factors in the absence of SA, and activates expression via TGA factors when sumoylated and SA is present.
• Essential for benzothiadiazole (BTH)-induced resistance against rice blast fungus (Magnaporthe oryzae). Related references: PMID:20924648
• Contributes to defense against bacterial blight (Xanthomonas oryzae pv. oryzae); overexpression enhances disease resistance but increases herbivore susceptibility. Related references: PMID:17309686
• Exhibits transcriptional coactivator activity with TGA2.1 and LG2 in vitro. Related references: PMID:22353606
• Modulates herbivore-induced defense responses; silencing reduces trypsin proteinase inhibitors and volatiles, enhancing resistance to Chilo suppressalis. Related references: PMID:22694163
• Localizes to the cytoplasm, nucleus, and nuclear bodies, consistent with its roles in signaling and transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen spans a region near the C-terminus (residues 475–582), which may be suitable for detecting full-length OsNPR1 in Western blotting under reducing conditions.
• For Western blot, consider using appropriate positive control lysates from rice (Oryza sativa) tissues known to express OsNPR1, such as leaves after pathogen or SA treatment.
• In ELISA, validate the antibody with the recombinant immunogen as a standard and test cross-reactivity with related NPR1-like proteins if needed.
• Since OsNPR1 undergoes sumoylation and phosphorylation, post-translational modifications may affect antibody recognition; verify in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with a streamlined procurement channel for IVD raw materials like this anti-OsNPR1 polyclonal antibody, essential for plant pathology and defense signaling studies. Beyond single antibodies, our catalog includes validated pairs, bulk reagents, and custom sourcing for rare targets, supporting your workflow from concept to clinic.