Anti-OSMR Polyclonal Antibody for WB, ELISA - Q99650

Core Product Specifications and Parameters

Biological Background: OSMR Function and Localization

• OSMR serves as a shared signal-transducing subunit in two receptor complexes: the type II oncostatin M (OSM) receptor (heterodimer with IL6ST) and the interleukin-31 (IL-31) receptor (heterodimer with IL31RA), binding their respective ligands OSM and IL-31.
  Related references: PMID:15184896, PMID:15627637, PMID:8999038

• OSM/IL6ST/OSMR signaling regulates cell proliferation, differentiation, and cytokine production, whereas IL-31/OSMR/IL31RA signaling is particularly critical for skin immunity.
  Related references: PMID:15184896, PMID:8999038

• Upon ligand binding, receptor dimerization activates associated kinases JAK1, JAK2, and to a lesser extent TYK2, which phosphorylate tyrosine residues on the receptor chains, creating docking sites for STAT1, STAT3, and STAT5B.
  Related references: PMID:15627637, PMID:8999038, PMID:9188471

• The IL-31 receptor complex additionally triggers the MAPK/ERK pathway (MAPK3/ERK1-MAPK1/ERK2) through recruitment of the adaptor protein SHC1.
  Related references: PMID:15627637

• OSMR is a single-pass type I transmembrane protein that undergoes alternative splicing, N‑linked glycosylation, and phosphorylation.

• It is expressed in keratinocytes, neural cells, fibroblasts, and epithelial cells, and has been implicated in conditions such as primary cutaneous amyloidosis.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 100–200 of human OSMR, located in the extracellular domain. This recombinant fragment may be suitable for detecting both membrane-bound and soluble receptor forms in Western blot and ELISA applications.
• For Western blot, the expected band is approximately 111 kDa (UniProt: 110.5 kDa). It is advisable to use positive controls such as OSMR-expressing cell lines (e.g., keratinocytes) and to optimize primary antibody dilution empirically.
• The antibody cross-reacts with mouse OSMR, enabling its use in murine model studies. Always verify reactivity and specificity in the specific tissue or cell type under investigation.
• As a polyclonal antibody, inter-lot consistency should be monitored; consider using blocking peptides or genetic knockout controls to confirm band identity in critical assays.
• For ELISA, titration of both coating antigen and detection antibody is recommended; starting concentrations in the range of 0.1–1 µg/mL may serve as a useful guideline.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your entire assay development pipeline. This anti-OSMR antibody addresses research needs in cytokine signaling, skin immunity, and cancer biology, offering a reliable tool for diagnostic manufacturers and labs. Partner with CamelBio to streamline your supply chain and accelerate progress from concept to clinic.