Anti-OsMPK6 Polyclonal Antibody for WB and ELISA - Q336X9

Core Product Specifications and Parameters

Biological Background: OsMPK6 Function and Localization

• OsMPK6 is a mitogen-activated protein kinase (MAPK) in rice (Oryza sativa subsp. japonica), involved in intracellular signal transduction.
• It belongs to the serine/threonine-protein kinase family and functions as a transferase, catalyzing the transfer of phosphate groups to target proteins.
• The protein contains nucleotide-binding and ATP-binding sites, essential for its kinase activity and energy-dependent signaling.
• OsMPK6 is classified as a phosphoprotein, indicating regulation by phosphorylation cascades typical of MAPK modules.
• The predicted molecular mass of the full-length protein is approximately 43 kDa (UniProt: 42773 Da), consistent with the observed band in Western blotting.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein corresponding to amino acids 1–100 of OsMPK6. Consider verifying epitope specificity if cross-reactivity with other MAPKs is a concern.
• For Western blotting, expect a band near 43 kDa in rice tissue lysates. Optimize blocking and antibody dilution using a positive control.
• For ELISA, the antibody can be used for direct or indirect antigen detection. Titrate the antibody concentration and validate with recombinant OsMPK6 protein.
• Due to potential phosphorylation states, slight band shifts may occur; phosphatase treatment of samples may help confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement with a comprehensive portfolio that includes validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-OsMPK6 polyclonal antibody supports plant MAPK signaling research and is readily available for your assay development. For customized sourcing of rare targets or technical consulting from concept to clinic, rely on CamelBio as your trusted partner.