Anti-OsMKK4 Polyclonal Antibody for WB, ELISA - Q6K4Q0

Core Product Specifications and Parameters

Biological Background: OsMKK4 Function and Localization

• OsMKK4 is a mitogen-activated protein kinase kinase (MAPKK) that functions as a serine/threonine-protein kinase, catalyzing the transfer of phosphate groups to downstream MAP kinases.
• It contains essential ATP-binding and nucleotide-binding domains, characteristic of active kinases involved in phosphorylation cascades.
• OsMKK4 is classified as a phosphoprotein, indicating that its activity is likely regulated by reversible phosphorylation.
• The protein is implicated in plant defense mechanisms, specifically associated with the hypersensitive response—a form of programmed cell death that restricts pathogen spread.
• It is part of the reference proteome for Oryza sativa and has been identified through proteomics studies.
• As a transferase, OsMKK4 plays a pivotal role in signal transduction pathways underlying stress and developmental responses in rice.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans amino acids 100-200 of OsMKK4, a region that may recognize both phosphorylated and unphosphorylated states; consider this when interpreting results.
• This polyclonal antibody has been tested for Western blot (WB) and ELISA with Oryza sativa samples; for other species or applications, validate performance in your assay system.
• For WB, consider using a loading control from rice and optimize antibody dilution and blocking conditions based on your sample preparation.
• When detecting OsMKK4, be mindful of its predicted molecular weight (~40 kDa) and potential post-translational modifications that may alter migration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials, including validated antibodies like this anti-OsMKK4 polyclonal antibody for research into plant kinase signaling and defense. As a one-stop sourcing partner, we support diagnostic manufacturers and labs with optimized antibody pairs, bulk ancillary reagents, and access to rare-target raw materials—empowering your development from concept to clinic. Contact our team to explore custom sourcing solutions tailored to your plant biology or kinase research needs.