Anti-OsMADS15 Rabbit Polyclonal Antibody for WB, ELISA - Q6Q9I2

Core Product Specifications and Parameters

Biological Background: OsMADS15 Function and Localization

• OsMADS15 (also known as RAP1A, FDRMADS3, RMADS215) is a MADS-box transcription factor encoded by the MADS15 gene in Oryza sativa (rice).
• It is a homolog of Arabidopsis APETALA1, a key regulator of floral meristem and organ identity.
• Functions as a probable transcription factor, binding DNA and regulating gene expression.
• Localizes to the nucleus, consistent with its role in transcriptional regulation.
• Belongs to the MADS-box family of transcription factors, characterized by a conserved DNA-binding domain.
• Predicted molecular weight of approximately 30 kDa, matching the observed protein weight of 30 kDa.
• Plays a potential role in rice flowering and reproductive development, as suggested by its similarity to APETALA1.
• Keywords: DNA-binding, Nucleus, Transcription regulation, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 167-267 of OsMADS15, likely outside the conserved MADS-box domain. This design may reduce cross-reactivity with other MADS-box proteins—validate specificity in your system using appropriate controls (e.g., knockout or overexpression lines).
• For Western blot (WB) analysis, consider enriching nuclear protein fractions from rice tissues to improve detection, given the nuclear localization of OsMADS15.
• ELISA has been validated for this antibody; consider pairing it with a suitable detection system for quantitative assays.
• Always optimize experimental conditions (e.g., antibody dilution, blocking buffers) for your specific sample type and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing diagnostic manufacturers and research labs with access to validated antibodies like anti-OsMADS15 for plant developmental studies. In addition to polyclonal antibodies, we supply optimized monoclonal antibodies, bulk ancillary reagents, and can source raw materials for rare targets, streamlining your workflow from concept to clinic.