Anti-OsMADS1 Rabbit Polyclonal Antibody for WB, ELISA - Q10PZ9

Core Product Specifications and Parameters

Biological Background: OsMADS1 Function and Localization

• OsMADS1 (also known as LEAFY HULL STERILE 1 or SEPALLATA-like) is a MADS-box transcription factor 1 in Oryza sativa (rice).
• It functions as a probable transcriptional activator involved in floral organ development, required for the formation of inner floral organs (lodicules, stamens, carpels) and the lemma/palea.
• OsMADS1 likely controls flowering time and acts upstream of the auxin-responsive protein GH3.8.
• It localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Tissue-specific expression is observed in lemmas, paleas, and pistils, with weaker expression in carpels.
• UniProt keywords highlight its roles as an activator, developmental protein, and its involvement in differentiation, flowering, and transcription regulation.
• The protein has a calculated molecular weight of approximately 29.5 kDa, with an observed weight around 30 kDa depending on modification context.

Experimental Guidance and Technical Tips

• For Western blotting (WB), using whole-cell lysates or nuclear extracts from Oryza sativa tissues (e.g., developing florets) is recommended; the expected band is around 30 kDa.
• Consider optimizing blocking and antibody dilution to minimize non-specific binding, common with polyclonal antibodies.
• For ELISA, the recombinant immunogen (aa 150-257) can be used as a coating antigen for titration and quality control experiments.
• Validate antibody specificity in the relevant sample system, such as by using a negative control (e.g., tissues with low OsMADS1 expression) or knockdown/mutant lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-OsMADS1 antibody is part of our extensive catalog supporting plant developmental biology and transcription factor research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials to streamline your IVD development.